This is my latest project of DNA extraction. Here i provide you the abstract of the project.
PROJECT #1
ABSTRACT: DNA EXTRACTION
BACKGROUND & OBJECTIVE
In this era, DNA is very common topic discussion and it is eminent as the secret of life. It is responsible to duplicate genetic information of every generation to another. DNA extraction is the basic introduction of observing DNA. This is the first skill of scientist to know DNA further by removing DNA from its cell.
QUESTIONS How does soap play role in DNA extraction?
HYPOTHESIS Soap, as a base chemical can removes lipids in cell membrane. The
more soap is used, the more DNA is visible.
OBJECTIVES The objective of this experiment is to know further every roles of experiment’s equipment, in this case is the purpose of soap.
METHODS
MATERIALS
Teaspoon (1)
Clear drinking glass (1)
Egg (1)
Cold alcohol (100 ml)
Plastic bag (1)
Coffee filter (1)
3 teaspoon of shower soap
PROCEDURES
STEPS OF EXPERIMENT #A
Beat the egg and scramble it in the clear drinking glass using tea spoon.
When it mixed well, add a teaspoon of shower soap.
Then mix the solution well.
Pour 100 ml of alcohol isopropyl into the glass.
Wait until white strands come up and float in the alcohol.
STEPS OF EXPERIMENT #B
Beat the egg and scramble it in the clear drinking glass using tea spoon.
When it mixed well, add two teaspoons of shower soap.
Then mix the solution well.
Pour 100 ml of alcohol isopropyl into the glass.
Wait until white strands come up and float in the alcohol.
RESULT
The white strands of egg’s DNA will float in the alcohol. Based on the experiments, experiment #B has shown more white strands of DNA than experiment #A. This is because soap, as a base chemical can removes lipids in cell membrane. The more soap is used, the more DNA is visible. Thus the more cells are losing its membrane; the more DNA strands are able to dissolve in alcohol isopropyl from its nucleus.
CONCLUSION
This experiment was conducted to observe the purpose of soap (base) in DNA extraction. DNA extraction is a very common experiment because it is the basic step of DNA’s voyage. Soap as a base chemical, holds an important role in rupturing DNA from the cell by removes the lipid in cell membranes.
SUMMARY STATEMENT
My project has shown the importance of base chemical in this experiment. A base chemical is able to remove the cell membrane’s lipid.
BIBLIOGRAPHY
http://apps.caes.uga.edu
http://www.youtube.com/watch?v=HgUgAeAb4Ng 06/25/2010
Labels: base, biology, cell, chemical, DNA, egg, experiment, genetic, lipid, membrane, project, purpose, role, soap
